Sampling Instructions for BeCrop Test

Identify a block with the same crop and management history.
Collect multiple cores using a “Z” or “W” pattern and mix them together.
Depth: 5–15 cm (2–6 inches).
Proximity:
- Fruit crops: 60 cm (24 in) from the trunk
- Vineyards & super-intensive systems: 30 cm (12 in) from the plant
- Row crops: in the root zone

Important: Avoid sampling for 3 days after heavy rainfall and do not sample directly under drip emitters.

BeCrop:
- Use the coded tube provided by Biome Makers (fill halfway, ~1–3 grams), or
- Use a sterile labeled bag (~1/4 cup or 10 grams minimum).

BeCrop+:
- Use the coded bag and fill to the line (~500 grams / 2 cups), or
- Use a labeled bag with equivalent volume.

Note: A mixing bucket is required for BeCrop+ sampling.

Include additional metadata (e.g., soil type, irrigation method) for more comprehensive analysis.

Ship samples immediately after collection to preserve microbial integrity.
Transit Time: Use 1- or 2-day shipping. If samples are in transit for more than 5 cumulative days at room temperature, you must include icepacks.
Short-Term Storage: Store samples at 0–6°C (32–48°F) for up to 3 days.
Long-Term Storage: Freeze at -20°C (-4°F) if shipping is delayed.

To ensure the highest accuracy when analyzing your soil microbiome, it's important to follow standardized sampling protocols. Proper collection and handling preserve soil DNA integrity and support accurate analysis of nutrient cycling, disease risk, and stress adaptation.