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BeCrop® Portal Tools: The Abundance BoxPlot Tool

The Abundance BoxPlot Tool lets you compare the relative abundance of specific microorganisms across groups of samples. Instead of looking at one sample (Explorer) or all metrics at once (HeatMap), the BoxPlot provides a deeper statistical view of how a particular microbe behaves under different conditions.

This tool is ideal for:

  • Comparing treated vs. untreated plots
  • Monitoring how a product affects microbial abundance (e.g., Trichoderma)
  • Tracking changes across parcels or timepoints
  • Quickly seeing differences between groups using min, max, and average values

 

Accessing the Abundance BoxPlot Tool

  1. Log into your BeCrop® Portal.
  2. From the left-hand menu, go to Toolbox.
  3. Select Abundance BoxPlot Tool.

 

Setting Up Clusters

The tool compares microorganisms across clusters of samples.

  1. Select samples: Click the small circle below each sample to assign it to a cluster.
    • Example: Assign control samples to Cluster 1 and treated samples to Cluster 2.

  2. Name clusters: Give each cluster a clear label (e.g., “Control untreated” vs. “Product treated”).

  3. Switch to name view (optional): Display sample names instead of codes for clarity.

  4. Save configurations: Use the disk icon to save your cluster setup.
    • Load saved configurations later via Load Saved Configurations.

 

Running a Comparison

1. After assigning samples, click Compare.



2. Select a microorganism from the dropdown menu, or type its name in the search bar.
    • Search by genus (e.g., Bacillus) to display all species within that group.
3. The tool will display results comparing your clusters.

 

Understanding Results

The chart helps you compare clusters using:

  • Average relative abundance
  • Minimum and maximum values (visible when hovering)
  • A clearer view as you add more samples to each cluster

Example Use Case

You are trialing a biological product and want to see whether Trichoderma increased:

  • Cluster 1: Control (untreated) samples
  • Cluster 2: Treated samples

You can quickly see whether treated samples show higher Trichoderma abundance than the control group.

When to Use This Tool

Use Abundance BoxPlot when you want to:

  • Focus on one microorganism
  • Compare groups of samples (plots, treatments, time periods)
  • Monitor changes in microbial abundance over time